locus

BSU10420

BSU_10420

geneLength

576

579

outlinks

bsu

BSU10420

BSU_10420

Gene

Coordinates

1,117,109 → 1,117,687

1,117,109 1,117,687

The protein

additional information

degraded by [[protein|ClpC]]-[[protein|ClpP]] (with [[protein|MecA]] as adaptor protein) [PubMed|9890793]

information on binding sites can be found in the [http://www.prodoric2.de/detail.php?acc=MX000023 PRODORIC2 database]

Biological materials

Mutant

1A871 (no resistance), [Pubmed| ], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A871&Search=1A871 BGSC]

BKE10420 (Δ[[gene|comK]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE10420 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATATTATGGCCTCCATCCT, downstream forward: _UP4_TAGAAAAATAGGAAGGAGCT

BKK10420 (Δ[[gene|comK]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK10420 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATATTATGGCCTCCATCCT, downstream forward: _UP4_TAGAAAAATAGGAAGGAGCT

1A871 (no resistance), [Pubmed| ], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A871&Search=1A871 BGSC]

BKE10420 ([[gene|comK]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE10420 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATATTATGGCCTCCATCCT, downstream forward: _UP4_TAGAAAAATAGGAAGGAGCT

BKK10420 ([[gene|comK]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK10420 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATATTATGGCCTCCATCCT, downstream forward: _UP4_TAGAAAAATAGGAAGGAGCT

Biological materials

Expression vector

for expression, purification in E. coli with N-terminal His-tag, pRSETA available in Gerth lab

Labs working on this gene/protein

[SW|Oscar Kuipers], University of Groningen, The Netherlands

[http://molgen.biol.rug.nl/molgen/index.php Homepage]

References

Reviews

19995980, 12576575, 26110607

19995980, 12576575, 26110607, 31950915

References

Original Publications

8878039, 11849533, 17569828, 9335269, 17560370, 12586407, 11814663, 9108277, 9890793, 9585513, 15819619, 10908654, 9000055, 7746142, 8830686, 10447896, 12028382, 8016067, 10361283, 11703662, 12107147, 17581123, 8016066, 17493123, 16391071, 16436435, 12270822, 15598897, 20300532, 12761164, 12036071, 21085679, 22517742, 7783616, 22412392, 8083168, 24838881, 8196543, 26110430, 27045827, 27920766, 28800172, 29124898

8878039, 11849533, 17569828, 9335269, 17560370, 12586407, 11814663, 9108277, 9890793, 9585513, 15819619, 10908654, 9000055, 7746142, 8830686, 10447896, 12028382, 8016067, 10361283, 11703662, 12107147, 17581123, 8016066, 17493123, 16391071, 16436435, 12270822, 15598897, 20300532, 12761164, 12036071, 21085679, 22517742, 7783616, 22412392, 8083168, 24838881, 8196543, 26110430, 27045827, 27920766, 28800172, 29124898, 29553055

The protein

Paralogous protein(s)

[[this]]

Biological materials

Expression vectors

for expression, purification in E. coli with N-terminal His-tag, pRSETA available in Gerth lab

labs

[SW|Oscar Kuipers], University of Groningen, The Netherlands

[http://molgen.biol.rug.nl/molgen/index.php Homepage]